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KMID : 0861420110150020116
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Korean Journal of Nuclear Medicine Technology
2011 Volume.15 No. 2 p.116 ~ p.124
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The Evaluation of Proficiency Test between Radioimmunoassay and Chemiluminescence Immunoassay
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Noh Kyeong-Woon
Kim Tae-Hoon
Kim Ji-Young
Kim Hyun-Joo
Lee Ho-Young
Choi Joon-Young
Lee Byoeng-Il
Choe Jae-Gol
Lee Dong-Soo
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Abstract
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Purpose:To establish accurate external quality assurance (EQA) test, cross institutional and modality tests were
performed using WHO certificated reference material (CRM) and same pooled patients serum. Materials and
Methods:Accuracy and precision were evaluated using CRM and pooled patients’ serum for AFP, CEA, PSA,
CA 125, CA 19-9, T3, T4, Tg, TSH. To evaluate the accuracy and precision, recover test and coefficient variation
were measured. RIA test were performed in major 5 RIA laboratory and EIA (CLIA) test were done in 5 major
EIA laboratory. same sample of CRM and pooled serum were delivered to each laboratory.
Results:In 2009,mean precision of total tumor marker of RIA was 14.8±4.2% and that of EIA(CLIA) was 19.2±6.9%. In 2010, mean precision of 5 tumor marker and T3, T4, Tg, TSH was 13.8±6.1% in RIA and 15.5±7.7% in EIA (CLIA).
There was no significant difference between RIA and EIA. In RIA, the coefficient variations (CV) of AFP, CEA,
PSA, CA 125, T3, T4, TSH were within 20%. The CV of CA 19-9 was over 20% but there was no significant
difference with EIA (CLIA) (p=0.345). In recovery test using CRM, AFP, PSA, T4, TSH showed 92~103% of
recovery in RIA. In recovery test using commercial material, CEA, CA 125, CA 19-9 showed relatively lower
recovery than CRM but there was no significant difference between RIA and EIA (CLIA).
Conclusion:By evaluating the precision and accuracy of each test, EQA test could more accurately measured the quality of each test and performance of laboratory.
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KEYWORD
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External Quality Assurance, Precision, Recovery test, CRM (certified reference material)
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